Histone H3 | HUABIO HA610203 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3

catalog :

HA610203

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

6-A7-R

reactivity :

human, mouse, rat

application :

western blot, chromatin immunoprecipitation, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (HA610203) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: A549 cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: PC-12 cell lysate Lane 5: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 14 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610203) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (HA610203) at 1/5,000 dilution. Lane 1: Rat testis tissue lysate Lane 2: Mouse skin tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 15 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610203) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-Histone H3 antibody (HA610203) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610203

Target name :

Histone H3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P,IF-Tissue,ChIP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Histone H3 aa 1-50 / 136.

Uniprot id :

P68431>SwissProt: P68431 human;SwissProt: P84243 human;SwissProt: Q16695 human;SwissProt: Q6NXT2 human;SwissProt: Q71DI3 human;SwissProt: P68433 mouse;SwissProt: P84228 mouse;SwissProt: Q6LED0 rat

Host :

Mouse

Clone number :

6-A7-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, A549 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, C6 cell lysate, rat testis tissue lysate, mouse skin tissue lysate, human liver tissue, mouse epididymis tissue, mouse testis tissue, rat brain tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:1,000 ;IF-Tissue: 1:200 ;ChIP: Use 0.5~2 μg for 25 μg of chromatin.

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610203_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse epididymis tissue with Mouse anti-Histone H3 antibody (HA610203) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610203_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Mouse anti-Histone H3 antibody (HA610203) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610203_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Histone H3 antibody (HA610203) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610203) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA610203_7.jpg

Pic legend7 :

Immunofluorescence analysis of paraffin-embedded human breast cancer tissue labeling Histone H3 (HA610203, red) and HER2 / ErbB2 (HA721210, green). The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS. And then probed with the primary antibodies Histone H3 (HA610203, red) at 1/200 dilution and HER2 / ErbB2 (HA721210, green) at 1/200 dilution overnight at 4 ℃, washed with PBS. iFluor™ 594 conjugate-Goat anti-Mouse IgG (HA1126) and iFluor™ 488 conjugate-Goat anti-Rabbit IgG (HA1121) were used as the secondary antibodies at 1/1,000 dilution. DAPI was used as nuclear counterstain.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA610203_8.jpg

Pic legend8 :

Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells with Histone H3 (HA610203) or Normal Mouse IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

more info or order :

HUABIO product webpage