EIF3H | HUABIO HA610200 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

EIF3H

catalog :

HA610200

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH04-49

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of EIF3H on different lysates with Mouse anti-EIF3H antibody (HA610200) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: HeLa cell lysate Lane 3: K-562 cell lysate Lane 4: Jurkat cell lysate Lane 5: HepG2 cell lysate Lane 6: A549 cell lysate Lane 7: SK-Br-3 cell lysate Lane 8: MDA-MB-231 cell lysate Lane 9: MDA-MB-468 cell lysate Lane 10: MCF7 cell lysate Lane 11: RAW264.7 cell lysate Lane 12: NIH/3T3 cell lysate Lane 13: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610200) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-EIF3H antibody (HA610200) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610200) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon cancer tissue with Mouse anti-EIF3H antibody (HA610200) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610200) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610200

Target name :

EIF3H

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human EIF3H aa 1-352 / 352.

Uniprot id :

O15372>SwissProt: O15372 Human;SwissProt: Q91WK2 Mouse;SwissProt: Q6P9U8 Rat

Host :

Mouse

Clone number :

PSH04-49

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HEK-293 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, HepG2 cell lysate, A549 cell lysate, SK-Br-3 cell lysate, MDA-MB-231 cell lysate, MDA-MB-468 cell lysate, MCF7 cell lysate, RAW264.7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, human breast cancer tissue, human colon cancer tissue.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC-P: 1:200

more info or order :

HUABIO product webpage