ALDH4A1 | HUABIO HA610192 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ALDH4A1

catalog :

HA610192

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7E2-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ALDH4A1 on different lysates with Mouse anti-ALDH4A1 antibody (HA610192) at 1/5,000 dilution. Lane 1: HepG2 cell lysate (20 µg/Lane) Lane 2: SK-Br-3 cell lysate (20 µg/Lane) Lane 3: Human kidney tissue lysate (40 µg/Lane) Lane 4: Human liver tissue lysate (40 µg/Lane) Lane 5: Mouse liver tissue lysate (40 µg/Lane) Lane 6: Rat liver tissue lysate (40 µg/Lane) Predicted band size: 62 kDa Observed band size: 55 kDa Exposure time: 1 minute 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610192) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HepG2 cells labeling ALDH4A1 with Mouse anti-ALDH4A1 antibody (HA610192) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-ALDH4A1 antibody (HA610192) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Counterstained with Mitotracker. Nuclear DNA was labelled in blue with DAPI.

image 3 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-ALDH4A1 antibody (HA610192) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610192) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610192

Target name :

ALDH4A1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human ALDH4A1 aa 514-563 / 563.

Uniprot id :

P30038>SwissProt: P30038 Human;SwissProt: Q8CHT0 Mouse;SwissProt: P0C2X9 Rat

Host :

Mouse

Clone number :

7E2-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HepG2 cell lysate, SK-Br-3 cell lysate, human kidney tissue lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, HepG2, human kidney tissue, human liver tissue, mouse liver tissue, rat liver tissue.

Molecular wt :

Predicted band size: 62 kDa

Subcellular location :

Mitochondrion matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610192_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-ALDH4A1 antibody (HA610192) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610192) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610192_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue with Mouse anti-ALDH4A1 antibody (HA610192) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610192) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610192_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-ALDH4A1 antibody (HA610192) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610192) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage