ESD | HUABIO HA610178 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ESD

catalog :

HA610178

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

J0-8-R

reactivity :

human, mouse, rat

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA610178) at 1/1,000 dilution. Lane 1: K-562 cell lysate Lane 2: Jurkat cell lysate Lane 3: HeLa cell lysate Lane 4: HepG2 cell lysate Lane 5: SW480 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 13 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610178) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA610178) at 1/2,000 dilution. Lane 1: Human kidney tissue lysate Lane 2: Mouse kidney tissue lysate Lane 3: Mouse colon tissue lysate Lane 4: Mouse stomach tissue lysate Lane 5: Rat kidney tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat stomach tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute 18 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610178) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of ESD on different lysates with Mouse anti-ESD antibody (HA610178) at 1/20,000 dilution. Lane 1: HAP1-parental cell lysate Lane 2: HAP1-ESD KD cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610178) at 1/20,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA610178

Target name :

ESD

Species reactivity :

Human,Mouse,Rat

Applications :

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ESD aa 1-182 / 282.

Uniprot id :

P10768>SwissProt: P10768 Human;SwissProt: Q9R0P3 Mouse;SwissProt: B0BNE5 Rat

Host :

Mouse

Clone number :

J0-8-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

K-562 cell lysate, Jurkat cell lysate, HeLa cell lysate, HepG2 cell lysate, SW480 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse colon tissue lysate, mouse stomach tissue lysate, rat kidney tissue lysate, rat colon tissue lysate, rat stomach tissue lysate.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cytoplasm. Cytoplasmic vesicle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:20,000

Advanced Validation :

Knockdown (KD)

more info or order :

HUABIO product webpage