Human CD45 | HUABIO HA610158 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CD45

catalog :

HA610158

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH03-64

reactivity :

human

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of Raji cells labeling Human CD45 with Mouse anti-Human CD45 antibody (HA610158) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Human CD45 antibody (HA610158) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 2 :

Flow cytometric analysis of human PBMC labeling Human CD45. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA610158, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA610158

Target name :

Human CD45

Species reactivity :

Human

Applications :

IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide

Uniprot id :

P08575>SwissProt: P08575 Human

Host :

Mouse

Clone number :

PSH03-64

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Raji, human PBMC.

Molecular wt :

Predicted band size: 147 kDa

Subcellular location :

Cell membrane, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100-1:1,000 ;FC: 1:1,000

more info or order :

HUABIO product webpage