Histone H3 | HUABIO HA610153 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Histone H3

catalog :

HA610153

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A11-D7-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Histone H3 on different lysates with Mouse anti-Histone H3 antibody (HA610153) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: A431 cell lysate Lane 4: MCF7 cell lysate Lane 5: NIH/3T3 cell lysate Lane 6: C2C12 cell lysate Lane 7: C6 cell lysate Lane 8: PC-12 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 15 kDa Observed band size: 15 kDa Exposure time: 25 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610153) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human skin tissue with Mouse anti-Histone H3 antibody (HA610153) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610153) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Histone H3 antibody (HA610153) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610153) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610153

Target name :

Histone H3

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within N-terminal human Histone H3.

Uniprot id :

P68431>SwissProt: P68431 human;SwissProt: P84243 human;SwissProt: Q16695 human;SwissProt: Q6NXT2 human;SwissProt: Q71DI3 human;SwissProt: P68433 mouse;SwissProt: P84228 mouse;SwissProt: Q6LED0 rat

Host :

Mouse

Clone number :

A11-D7-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, A431 cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, PC-12 cell lysate, human skin tissue, mouse brain tissue, rat testis tissue.

Molecular wt :

Predicted band size: 15 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000 ;IHC-P: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610153_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat testis tissue with Mouse anti-Histone H3 antibody (HA610153) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610153) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage