GST3 | HUABIO HA610147 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

GST3

catalog :

HA610147

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

11D5-R

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of GST3 on different lysates with Mouse anti-GST3 antibody (HA610147) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: HEK-293 cell lysate Lane 3: MDA-MB-231 cell lysate Lane 4: HT-29 cell lysate Lane 5: Jurkat cell lysate Lane 6: K-562 cell lysate Lane 7: JAR cell lysate Lane 8: Human brain tissue lysate Lane 9: Human lung tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 1 minute 2 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610147) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HT-29 cells labeling GST3 with Mouse anti-GST3 antibody (HA610147) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-GST3 antibody (HA610147) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human testis tissue with Mouse anti-GST3 antibody (HA610147) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610147) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610147

Target name :

GST3

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human GST3 aa 1-183 / 210.

Uniprot id :

P09211>SwissProt: P09211 Human

Host :

Mouse

Clone number :

11D5-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, HEK-293 cell lysate, MDA-MB-231 cell lysate, HT-29 cell lysate, Jurkat cell lysate, K-562 cell lysate, JAR cell lysate, human brain tissue lysate, human lung tissue lysate, HT-29, human testis tissue, human kidney tissue, human lung cancer tissue.

Molecular wt :

Predicted band size: 23 kDa

Subcellular location :

Nucleus, Cytoplasm, Mitochondrion.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:5,000

Advanced Validation :

Knockdown (KD)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610147_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human kidney tissue with Mouse anti-GST3 antibody (HA610147) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610147) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610147_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-GST3 antibody (HA610147) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610147) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610147_6.jpg

Pic legend6 :

Western blot analysis of GST3 on different lysates with Mouse anti-GST3 antibody (HA610147) at 1/1,000 dilution. Lane 1: HCT 116-si NT cell lysate Lane 2: HCT 116-si GST3 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 23 kDa Observed band size: 23 kDa Exposure time: 3 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610147) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage