product summary
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company name :
HUABIO
product type :
antibody
product name :
BrdU
catalog :
HA610099
quantity :
100μg
price :
649.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PSH0-18
application :
immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunohistochemical analysis of paraffin-embedded mouse embryo brain tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA610099) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610099) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 2 :
Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA610099) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610099) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
image 3 :
Immunohistochemical analysis of paraffin-embedded mouse embryo liver tissue (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA610099) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610099) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA610099
Target name :
BrdU
Species reactivity :
Species independent
Applications :
IHC-P,IF-Cell,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
BrdU-OVA
Uniprot id :
>
Host :
rabbit
Clone number :
PSH0-18
Isotype :
IgG1
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
BrdU treated mouse embryo brain tissue, BrdU treated mouse embryo cartilage tissue, BrdU treated mouse embryo liver tissue, BrdU treated NIH/3T3.
Subcellular location :
Nucleus.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:10,000
;IF-Cell: 1:50
;IF-Tissue: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA610099_4.jpg
Pic legend4 :
Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells (Brdu treated / Untreated / Edu treated) with Mouse anti-BrdU antibody (HA610099) at 1/10,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610099) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA610099_5.jpg
Pic legend5 :
Immunocytochemistry analysis of NIH/3T3 cells (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA610099) at 1/50 dilution.
Cells were fixed in 70% ethyl alcohol for 5 minutes at room temperature, then subjected to acid hydrolysis using 2M HCL in TBST for 30 minutes at room temperature. permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and then blocked with 2% BSA for 30 minutes at room temperature. Cells were then incubated with Mouse anti-BrdU antibody (HA610099) at 1/50 dilution in 2% negative goat serum overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.
Pic img6 :
https://storage.huabio.cn/huabio/productImg/HA610099_6.jpg
Pic legend6 :
Immunofluorescence analysis of paraffin-embedded mouse embryo brain tissue (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA610099) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610099, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
Pic img7 :
https://storage.huabio.cn/huabio/productImg/HA610099_7.jpg
Pic legend7 :
Immunofluorescence analysis of paraffin-embedded mouse embryo cartilage tissue (Untreated / Brdu treated / Edu treated) labeling BrdU with Mouse anti-BrdU antibody (HA610099) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA610099, green) at 1/1,000 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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