product summary
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company name :
HUABIO
product type :
antibody
product name :
ZAP70
catalog :
HA610096
quantity :
100μg
price :
649.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A1B5-R
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - paraffin section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA610096 image 1
Western blot analysis of ZAP70 on different lysates with Mouse anti-ZAP70 antibody (HA610096) at 1/1,000 dilution. Lane 1: Jurkat cell lysate Lane 2: Ramos cell lysate (negative) Lane 3: Mouse spleen tissue lysate Lane 4: Mouse thymus tissue lysate Lane 5: Rat spleen tissue lysate Lane 6: Rat thymus tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 70 kDa Observed band size: 70 kDa Exposure time: 1 minute 21 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610096) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
HUABIO HA610096 image 2
Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-ZAP70 antibody (HA610096) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610096) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA610096
Target name :
ZAP70
Species reactivity :
Human,Mouse,Rat
Applications :
WB,IHC-P
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ZAP70 aa 250-480.
Uniprot id :
P43403>SwissProt: P43403 Human;SwissProt: P43404 Mouse;Entrez Gene: 301348 Rat
Host :
Mouse
Clone number :
A1B5-R
Isotype :
IgG1
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
Jurkat cell lysate, mouse spleen tissue lysate, mouse thymus tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate, human tonsil tissue.
Molecular wt :
Predicted band size: 70 kDa
Subcellular location :
Cell membrane, cytoplasm.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000 ;IHC-P: 1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!