Peroxiredoxin 2 | HUABIO HA610082 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Peroxiredoxin 2

catalog :

HA610082

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

7F5-R

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Peroxiredoxin 2 on different lysates with Mouse anti-Peroxiredoxin 2 antibody (HA610082) at 1/1,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: LNCaP cell lysate Lane 3: HeLa cell lysate Lane 4: SH-SY5Y cell lysate Lane 5: MCF7 cell lysate Lane 6: HepG2 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 5 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610082) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling Peroxiredoxin 2 with Mouse anti-Peroxiredoxin 2 antibody (HA610082) at 1/100 dilution. Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Peroxiredoxin 2 antibody (HA610082) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (HA610082) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610082

Target name :

Peroxiredoxin 2

Species reactivity :

Human

Applications :

WB,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant full length protein.

Uniprot id :

P32119>SwissProt: P32119 Human

Host :

Mouse

Clone number :

7F5-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HEK-293 cell lysate, LNCAP cell lysate, HeLa cell lysate, SH-SY5Y cell lysate, MCF7 cell lysate, HepG2 cell lysate, HeLa, human breast carcinoma tissue, human liver carcinoma tissue, human thyroid carcinoma tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cytoplasm

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:100 ;IHC-P: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610082_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (HA610082) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610082_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue with Mouse anti-Peroxiredoxin 2 antibody (HA610082) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610082) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage