product summary
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company name :
HUABIO
product type :
antibody
product name :
ERK2
catalog :
HA610079
quantity :
100μg
price :
649.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
C3B12-R
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA610079) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: Jurkat cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: A431 cell lysate (20 µg/Lane)
Lane 5: HepG2 cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: NIH/3T3 cell lysate (20 µg/Lane)
Lane 8: RAW264.7 cell lysate (20 µg/Lane)
Lane 9: C6 cell lysate (20 µg/Lane)
Lane 10: Human brain tissue lysate (40 µg/Lane)
Lane 11: Mouse brain tissue lysate (40 µg/Lane)
Lane 12: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 43 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610079) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Alpaca Anti-Mouse IgG - HRP for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of ERK2 on different lysates with Mouse anti-ERK2 antibody (HA610079) at 1/2,000 dilution.
Lane 1: A549-si NT cell lysate (10 µg/Lane)
Lane 2: A549-si ERK2 cell lysate (10 µg/Lane)
Predicted band size: 41 kDa
Observed band size: 41 kDa
Exposure time: 1 minute; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610079) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA610079
Target name :
ERK2
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human ERK2 aa 200-360.
Uniprot id :
P28482>SwissProt: P28482 Human;SwissProt: P63085 Mouse;SwissProt: P63086 Rat
Host :
Mouse
Clone number :
C3B12-R
Isotype :
IgG1
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, Jurkat cell lysate, A549 cell lysate, A431 cell lysate, HepG2 cell lysate, HEK-293 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate, human brain tissue lysate, mouse brain tissue lysate, rat brain tissue lysate.
Molecular wt :
Predicted band size: 41 kDa
Subcellular location :
Cytoplasm, cytoskeleton, spindle, Nucleus, microtubule organizing center, centrosome, Membrane, caveola, Cell junction, focal adhesion.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000-1:2,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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