antibody

DYKDDDDK Tag (FLAG)

HA610054

100μg

649.00 USD

monoclonal

mouse

nonconjugated

A2-A4-R

western blot, immunoprecipitation, immunohistochemistry - paraffin section

DYKDDDDK Tag (FLAG)

Species independent

WB,IP,IF-Cell,IHC-P

Non-conjugated

Synthetic peptide immune sequence is N-DYKDDDDK-C.

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Mouse

A2-A4-R

IgG1

100μg

649.00 USD

PBS (pH7.4).

Liquid

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Protein A affinity purified.

Recombinant Mouse monoclonal Antibody

1 mg/mL.

WB: 1:5,000-1:10,000 ;IP: 2-5 µg/ml. ;IF-Cell: 1:250-1:10,000 ;IHC-P: 1:1,000

https://storage.huabio.cn/huabio/productImg/HA610054_4.jpg

DYKDDDDK Tag (FLAG) was immunoprecipitated in 2µg L-929 transfected with FLAG-tagged CD5 (C-terminal) cell lysate with HA610054. Western blot was performed from the immunoprecipitate using DYKDDDDK Tag(FLAG) (M1403-2) at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-Secondary Antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: L-929 transfected with FLAG-tagged CD5 (C-terminal) cell lysate (input). Lane 2: Mouse IgG instead of HA610054 IP in L-929 transfected with FLAG-tagged CD5 (C-terminal) cell lysate. Lane 3: HA610054 IP in L-929 transfected with FLAG-tagged CD5 (C-terminal) cell lysate. Blocking/Dilution buffer: 5% NFDM/TBST.

https://storage.huabio.cn/huabio/productImg/HA610054_5.jpg

Immunohistochemical analysis of paraffin-embedded HeLa transfected with FLAG-tagged Histon H3.1 (N-terminal) cells with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA610054) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610054) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA610054_6.jpg

Immunohistochemical analysis of paraffin-embedded HeLa transfected with FLAG-tagged Claudin 18.2 (C-terminal) cells with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA610054) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610054) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

https://storage.huabio.cn/huabio/productImg/HA610054_7.jpg

Immunocytochemistry analysis of HeLa cells labeling DYKDDDDK Tag (FLAG) with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA610054) at 1/250 dilution. HeLa cells, transfected with FLAG-tagged empty control, Claudin 18.2 (C-terminal) or Histone H3.1 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-DYKDDDDK Tag (FLAG) antibody (HA610054) at 1/250 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.