CD204 | HUABIO HA610052 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD204

catalog :

HA610052

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8G2-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD204 on different lysates with Mouse anti-CD204 antibody (HA610052) at 1/1,000 dilution. Lane 1: THP-1 treated with 50ng/mL PMA for 72 hours whole cell lysate Lane 2: THP-1 whole cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 50 kDa Observed band size: 75 kDa Exposure time: 50 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610052) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of THP-1 cells treated with or without 50ng/mL PMA for 72 hours labeling CD204 with Mouse anti-CD204 antibody (HA610052) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-CD204 antibody (HA610052) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-CD204 antibody (HA610052) at 1μg/mL dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610052) at 1μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610052

Target name :

CD204

Species reactivity :

Human,Rat

Applications :

WB,IHC-P,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD204 aa 51-250 / 451.

Uniprot id :

P21757>SwissProt: P21757 Human;SwissProt: P30204 Mouse;Entrez Gene: 498638 Rat

Host :

Mouse

Clone number :

A8G2-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

THP-1 treated with 50ng/mL PMA for 72 hours whole cell lysate, human liver tissue, human placenta tissue, human spleen tissue, rat liver tissue.

Molecular wt :

Predicted band size: 50 kDa

Subcellular location :

Membrane

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1ug/mL-5ug/mL ;IF-Cell: 1:100

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610052_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-CD204 antibody (HA610052) at 1μg/mL dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610052) at 1μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610052_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human spleen tissue with Mouse anti-CD204 antibody (HA610052) at 1μg/mL dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610052) at 1μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610052_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue with Mouse anti-CD204 antibody (HA610052) at 5μg/mL dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610052) at 5μg/mL dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage