E-Cadherin | HUABIO HA610047 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

E-Cadherin

catalog :

HA610047

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A0-G11-2-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of E-Cadherin on different lysates with Mouse anti-E-Cadherin antibody (HA610047) at 1/1,000 dilution. Lane 1: A431 cell lysate (10 µg/Lane) Lane 2: SW480 cell lysate (10 µg/Lane) Lane 3: MCF7 cell lysate (10 µg/Lane) Predicted band size: 98 kDa Observed band size: 130 kDa Exposure time: 20 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610047) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610047

Target name :

E-Cadherin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mouse E-Cadherin aa 350-550.

Uniprot id :

P12830>SwissProt: P12830 Human;SwissProt: P09803 Mouse;SwissProt: Q9R0T4 Rat

Host :

Mouse

Clone number :

A0-G11-2-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

A431 cell lysate, SW480 cell lysate, MCF7 cell lysate, human breast carcinoma tissue, human liver cancer tissue, human liver tissue, human lung cancer tissue, rat kidney tissue.

Molecular wt :

Predicted band size: 98 kDa

Subcellular location :

Cell membrane, Endosome, Golgi apparatus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:2,000 ;IHC: 1:200-1:10,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610047_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610047_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human lung cancer tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610047_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat kidney tissue with Mouse anti-E-Cadherin antibody (HA610047) at 1/10,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610047) at 1/10,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage