NeuN | HUABIO HA610031 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

NeuN

catalog :

HA610031

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PD01-45

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 (NeuN, HA610031, Mouse, green); 1/500 (Iba1, HA601368, Guinea pig, red) Antigen retrieval: Not required

image 2 :

Western blot analysis of NeuN on mouse cerebellum tissue lysates with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. Lysates/proteins at 20 µg/Lane. Predicted band size: 34 kDa Observed band size: 45/50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610031) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of NeuN on different lysates with Mouse anti-NeuN antibody (HA610031) at 1/500 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: SHG44 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 34 kDa Observed band size: 50 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610031) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA610031

Target name :

NeuN

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Tissue,IHC-P,IHC-Fr

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human NeuN aa 20-60.

Uniprot id :

A6NFN3>SwissProt: A6NFN3 Human;SwissProt: Q8BIF2 Mouse;Unigene:143966 Rat

Host :

Mouse

Clone number :

PD01-45

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

SH-SY5Y cell lysate, SHG44 cell lysate, Human brain tissue, mouse hippocampus tissue, mouse brain tissue, mouse cerebellum tissue, rat hippocampus tissue, rat brain tissue, rat cerebellum tissue, mouse cerebellum tissue lysates, mouse cerebral cortex tissue.

Molecular wt :

Predicted band size: 34 kDa

Subcellular location :

Cytoplasm, Nucleus

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Tissue: 1:500 ;IHC-P: 1:1,000 ;IHC-Fr: 1:500-1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610031_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human brain tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610031_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610031_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA610031_7.jpg

Pic legend7 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA610031_8.jpg

Pic legend8 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img9 :

https://storage.huabio.cn/huabio/productImg/HA610031_9.jpg

Pic legend9 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img10 :

https://storage.huabio.cn/huabio/productImg/HA610031_10.jpg

Pic legend10 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-NeuN antibody (HA610031) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610031) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img11 :

https://storage.huabio.cn/huabio/productImg/HA610031_11.jpg

Pic legend11 :

Application: IF-tissue Species: Mouse Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/500

Pic img12 :

https://storage.huabio.cn/huabio/productImg/HA610031_12.jpg

Pic legend12 :

Application: IF-tissue Species: Rat Site: Cerebral cortex Sample: Paraffin-embedded section Antibody concentration: 1/500

more info or order :

HUABIO product webpage