product summary
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company name :
HUABIO
product type :
antibody
product name :
beta Actin
catalog :
HA610019
quantity :
100μg
price :
649.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A2-F6-R
reactivity :
human, mouse, rat
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of beta Actin on different lysates with Mouse anti-beta Actin antibody (HA610019) at 1/40,000 dilution.
Lane 1: MCF-7 cell lysate (10 µg/Lane)
Lane 2: HepG2 cell lysate (10 µg/Lane)
Lane 3: THP-1 cell lysate (10 µg/Lane)
Lane 4: PMVEC cell lysate (10 µg/Lane)
Lane 5: RH-35 cell lysate (10 µg/Lane)
Lane 6: NIH/3T3 cell lysate (10 µg/Lane)
Lane 7: RAW264.7 cell lysate (10 µg/Lane)
Lane 8: B16F1 cell lysate (10 µg/Lane)
Lane 9: MEF cell lysate (10 µg/Lane)
Lane 10: Hela cell lysate (10 µg/Lane)
Lane 11: F9 cell lysate (10 µg/Lane)
Lane 12: PC-12 cell lysate (10 µg/Lane)
Lane 13: Mouse lung cell lysate (20 µg/Lane)
Lane 14: L6 cell lysate (10 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at 1/40,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of beta Actin on HepG2 cell lysates with Mouse anti-beta Actin antibody (HA610019) at different dilutions.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.
image 3 :
Western blot analysis of beta Actin on NIH/3T3 cell lysates with Mouse anti-beta Actin antibody (HA610019) at different dilutions.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 5 minutes;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA610019
Target name :
beta Actin
Species reactivity :
Human,Mouse,Rat
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within N-terminal human Beta-actin .
Uniprot id :
P60709>SwissProt: P60709 Human;SwissProt: P60710 Mouse;SwissProt: P60711 Rat
Host :
Mouse
Clone number :
A2-F6-R
Isotype :
IgG1
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, MCF-7 cell lysate, THP-1 cell lysate, PMVEC cell lysate, RH-35 cell lysate, RAW264.7 cell lysate, B16F1 cell lysate, MEF cell lysate, Hela cell lysate, F9 cell lysate, Mouse lung cell lysate, L6 cell lysate.
Molecular wt :
Predicted band size: 42 kDa
Subcellular location :
Cytoplasm
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:80,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA610019_4.jpg
Pic legend4 :
Western blot analysis of beta Actin on PC-12 cell lysates with Mouse anti-beta Actin antibody (HA610019) at different dilutions.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 1 minute;
10% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610019) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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