Myc tag | HUABIO HA610018 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Myc tag

catalog :

HA610018

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A3C8-R-2

application :

western blot, ELISA, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Myc tag on different lysates with Mouse anti-Myc tag antibody (HA610018) at 1/100,000 dilution. Lane 1: 293T transfected with Myc-tagged empty control cell lysate Lane 2: 293T transfected with Myc-tagged Claudin18.2 (C-terminal) cell lysate Lane 3: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate Lysates/proteins at 10 µg/Lane. Exposure time: 1 minute 2 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610018) at 1/100,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of 293T cells labeling Myc tag with Mouse anti-Myc tag antibody (HA610018) at 1/1,000 dilution. 293T cells, transfected with Myc-tagged empty control, Claudin18.2 (C-terminal) or Histone H3.1 (N-terminal) expression vector, respectively, were fixed in 4% paraformaldehyde for 10 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myc tag antibody (HA610018) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Myc Tag (R1208-1, red) was stained at 1/200 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

image 3 :

Myc-tag was immunoprecipitated in 2µg C-terminal Myc-tag fusion protein lysate with HA610018. Western blot was performed from the immunoprecipitate using HA610018 at 1/2,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1:5,000 dilution was used for 60 mins at room temperature. Lane 1: C-terminal Myc-tag fusion protein lysate (input). Lane 2: HA610018 IP in C-terminal Myc-tag fusion protein lysate. Lane 3: Mouse IgG instead of HA610018 IP in C-terminal Myc-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

SKU :

HA610018

Target name :

Myc tag

Species reactivity :

Species independent

Applications :

ELISA,WB,IP,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide EQKLISEEDL conjugated to keyhole limpet haemocyanin.

Uniprot id :

Host :

Mouse

Clone number :

A3C8-R-2

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Concentration :

1 mg/mL.

Recommended dilutions :

ELISA: 1:10,000 ;WB: 1:100,000-1:200,000 ;IP: Use at an assay dependent concentration. ;IF-Cell: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610018_4.jpg

Pic legend4 :

Myc-tag was immunoprecipitated in 2µg N-terminal Myc-tag fusion protein lysate with HA610018. Western blot was performed from the immunoprecipitate using HA610018 at 1/500 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1:2,000 dilution was used for 60 mins at room temperature. Lane 1: N-terminal Myc-tag fusion protein lysate (input). Lane 2: HA610018 IP in N-terminal Myc-tag fusion protein lysate. Lane 3: Mouse IgG instead of HA610018 IP in N-terminal Myc-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610018_5.jpg

Pic legend5 :

Myc tag Antibody (HA610018) in indirect ELISA. Indirect ELISA analysis of Myc tag was performed by coating wells of a 96-well plate with 50 µl per well of Myc tag antigen diluted in carbonate/bicarbonate buffer, at a concentration of 1 µg/mL overnight at 4℃. Wells of the plate were washed, blocked with StartingBlock blocking buffer, and incubated with 50 µl per well of a mouse Myc tag monoclonal antibody starting at a concentration of 20 µg/mL and serially diluting it to a concentration of 1.28 ng/mL for 1 hour at 37℃. The plate was washed and incubated with 50 µl per well of an HRP-conjugated goat anti-Mouse IgG secondary antibody at a dilution of 1:10,000 for 30 minutes at 37℃. Detection was performed using an Ultra TMB Substrate for 5 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

more info or order :

HUABIO product webpage