6X His tag | HUABIO HA610016 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

6X His tag

catalog :

HA610016

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A5D1-R

application :

western blot, immunoprecipitation

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of 6X His tag on different lysates with Mouse anti-6X His tag antibody (HA610016) at different dilutions. Lane 1/2: C-terminal His-tag fusion protein lysates Lane 3/4: Mid His-tag fusion protein lysates Lysates/proteins at 50 ng/Lane. Observed band size: 30/40 kDa Exposure time: 30 seconds; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610016) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

His-tag was immunoprecipitated in 2µg C-terminal His-tag fusion protein lysate with HA610016. Western blot was performed from the immunoprecipitate using HA610016 at 1/2,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 60 mins at room temperature. Lane 1: C-terminal His-tag fusion protein lysate (input). Lane 2: HA610016 IP in C-terminal His-tag fusion protein lysate. Lane 3: Mouse IgG instead of HA610016 IP in C-terminal His-tag fusion protein lysate. Blocking/Dilution buffer: 5% NFDM/TBST.

product information

SKU :

HA610016

Target name :

6X His tag

Species reactivity :

Species independent

Applications :

WB,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide HHHHHHGCGHHHHHH.

Uniprot id :

Host :

Mouse

Clone number :

A5D1-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

C-terminal His-tag fusion protein lysates, Mid His-tag fusion protein lysates.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:5,000-1:50,000 (C-His) ;WB: 1:1,000-1:5,000 (Mid-His) ;IP: Use at an assay dependent concentration (C-His)

more info or order :

HUABIO product webpage