product summary
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company name :
HUABIO
product type :
antibody
product name :
6X His tag (C-terminal)
catalog :
HA610015
quantity :
100μg
price :
649.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
8-F0-R
application :
western blot, immunoprecipitation
more info or order :
image
image 1 :
Western blot analysis of 6X His tag (C-terminal) on C-terminal His-tag fusion protein lysates with Mouse anti-6X His tag (C-terminal) antibody (HA610015) at different dilutions.
Lysates/proteins at 50 ng/Lane.
Observed band size: 30 kDa
Exposure time: 30 seconds;
12% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610015) at different dilutions was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.
image 2 :
His-tag was immunoprecipitated in 2µg C-terminal His-tag fusion protein lysate with HA610015. Western blot was performed from the immunoprecipitate using HA610015 at 1/2,000 dilution. Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 60 mins at room temperature.
Lane 1: C-terminal His-tag fusion protein lysate (input).
Lane 2: HA610015 IP in C-terminal His-tag fusion protein lysate.
Lane 3: Mouse IgG instead of HA610015 IP in C-terminal His-tag fusion protein lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
product information
SKU :
HA610015
Target name :
6X His tag (C-terminal)
Species reactivity :
Species independent
Applications :
WB,IP
Conjugate :
Non-conjugated
Immunogen :
C-terminal His-tagged recombinant protein, N-terminal His-tagged recombinant protein.
Uniprot id :
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Host :
Mouse
Clone number :
8-F0-R
Isotype :
IgG2a
Size :
100μg
List Price :
649.00 USD
Storage Buffer :
PBS (pH7.4).
Form :
Liquid
Storage Instruction :
Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
C-terminal His-tag fusion protein lysates.
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:5,000-1:20,000
;IP: Use at an assay dependent concentration.
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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