xCT/SLC7A11 | HUABIO HA610011 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

xCT/SLC7A11

catalog :

HA610011

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A7C6-R

reactivity :

human, mouse

application :

western blot, flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of xCT/SLC7A11 on different lysates with Mouse anti-xCT/SLC7A11 antibody (HA610011) at 1/500 dilution. Lane 1: HT-29 cell lysate Lane 2: A549 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 30 seconds; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of xCT/SLC7A11 on HCT116 cell lysates with Mouse anti-xCT/SLC7A11 antibody (HA610011) at 1/5,000 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 1 minute; 12% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610011) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Mouse anti-xCT/SLC7A11 antibody (HA610011) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610011) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610011

Target name :

xCT/SLC7A11

Species reactivity :

Human,Mouse

Applications :

WB,IHC-P,IF-Cell,FC

Conjugate :

Non-conjugated

Immunogen :

293 cell line overexpressing SLC7A11.

Uniprot id :

Q9UPY5>SwissProt: Q9UPY5 Human;SwissProt: Q9WTR6 Mouse

Host :

Mouse

Clone number :

A7C6-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HT-29 cell lysate, A549 cell lysate, HCT116 cell lysates, human pancreas tissue, HT-29, NIH/3T3 cell lysates.

Molecular wt :

Predicted band size: 55 kDa

Subcellular location :

Cell membrane, Cell projection, microvillus membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:5,000 ;IHC-P: 1:200 ;IF-Cell: 1:100-1:200 ;FC: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610011_4.jpg

Pic legend4 :

Immunocytochemistry analysis of HT-29 cells labeling xCT/SLC7A11 with Mouse anti-xCT/SLC7A11 antibody (HA610011) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-xCT/SLC7A11 antibody (HA610011) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) was used as the secondary antibody at 1/1,000 dilution.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA610011_5.jpg

Pic legend5 :

Flow cytometric analysis of HT-29 cells labeling xCT/SLC7A11. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA610011, 1/1,000) (red) compared with Mouse IgG1 Isotype Control (green). After incubation of the primary antibody at +4℃ for 30 minutes, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA610011_6.jpg

Pic legend6 :

Western blot analysis of xCT/SLC7A11 on NIH/3T3 cell lysates with Mouse anti-xCT/SLC7A11 antibody (HA610011) at 1/500 dilution. Lysates/proteins at 10 µg/Lane. Predicted band size: 55 kDa Observed band size: 55 kDa Exposure time: 1 minute; 8% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610011) at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:150,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage