CD133 | HUABIO HA610009 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD133

catalog :

HA610009

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8C3-R

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA610009) at 1/1,000 dilution. Lane 1: Caco-2 cell lysate Lane 2: HT-29 cell lysate Lane 3: HeLa cell lysate (negative) Lane 4: NCCIT cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of CD133 on different lysates with Mouse anti-CD133 antibody (HA610009) at 1/5,000 dilution. Lane 1: HT-29 cell lysate Lane 2: SW480 cell lysate Lane 3: NCCIT cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 97 kDa Observed band size: 120 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610009) at 1/5,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:100,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue with Mouse anti-CD133 antibody (HA610009) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610009) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610009

Target name :

CD133

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human CD133 aa 151-400/865.

Uniprot id :

O43490>SwissProt: O43490 Human

Host :

Mouse

Clone number :

A8C3-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Caco-2 cell lysate, HT-29 cell lysate, NCCIT cell lysate, SW480 cell lysate, human colon carcinoma tissue.

Molecular wt :

Predicted band size: 97 kDa

Subcellular location :

Endoplasmic reticulum. Plasma membrane. Cell projection.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage