KRAS | HUABIO HA610008 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

KRAS

catalog :

HA610008

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A8E6

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of KRAS on different lysates with Mouse anti-KRAS antibody (HA610008) at 1/2,000 dilution. Lane 1: HEK-293 cell lysate Lane 2: 22RV1 cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse kidney tissue lysate Lane 5: Rat brain tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 22 kDa Observed band size: 22 kDa Exposure time: 43 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610008) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse colon tissue with Mouse anti-KRAS antibody (HA610008) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610008) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue with Mouse anti-KRAS antibody (HA610008) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610008) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610008

Target name :

KRAS

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human KRAS aa 2-186.

Uniprot id :

P01116>SwissProt: P01116 Human;SwissProt: P32883 Mouse;SwissProt: P08644 Rat

Host :

Mouse

Clone number :

A8E6

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HEK-293 cell lysate, 22RV1 cell lysate, mouse brain tissue lysate, mouse kidney tissue lysate, rat brain tissue lysate, mouse colon tissue, mouse kidney tissue, rat colon tissue.

Molecular wt :

Predicted band size: 22 kDa

Subcellular location :

Cell membrane. Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:500-1:2,000 ;IHC-P: 1:1,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610008_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat colon tissue with Mouse anti-KRAS antibody (HA610008) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610008) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage