ROBO1 | HUABIO HA610007 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

ROBO1

catalog :

HA610007

quantity :

100μg

price :

649.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

10E2-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (HA610007) at 1/1,000 dilution. Lane 1: HeLa cell lysate (15 µg/Lane) Lane 2: Rat brain tissue lysate (40 µg/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610007) at 1/1,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of ROBO1 on different lysates with Mouse anti-ROBO1 antibody (HA610007) at 1/1,000 dilution. Lane 1: MCF-7 cell lysate (10 µg/Lane) Lane 2: NIH/3T3 cell lysate (10 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: PC-12 cell lysate (10 µg/Lane) Lane 5: RAW264.7 cell lysate (10 µg/Lane) Predicted band size: 181 kDa Observed band size: 181 kDa Exposure time: 30 seconds; 6% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA610007) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/300,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-ROBO1 antibody (HA610007) at 1/600 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610007) at 1/600 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA610007

Target name :

ROBO1

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ROBO1 aa 51-300 / 1,651.

Uniprot id :

Q9Y6N7>SwissProt: Q9Y6N7 Human;SwissProt: O89026 Mouse;SwissProt: O55005 Rat

Host :

Mouse

Clone number :

10E2-R

Isotype :

IgG1

Size :

100μg

List Price :

649.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, Rat brain tissue lysate, MCF-7 cell lysate, NIH/3T3 cell lysate, PC-12 cell lysate, RAW264.7 cell lysate, human breast tissue, human placenta tissue.

Molecular wt :

Predicted band size: 181 kDa

Subcellular location :

Cell membrane, Cell projection, axon, Endoplasmic reticulum-Golgi intermediate compartment membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:200-1:600

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA610007_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human placenta tissue with Mouse anti-ROBO1 antibody (HA610007) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA610007) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage