PGP9.5 | HUABIO HA601515 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

PGP9.5

catalog :

HA601515

quantity :

100μl

price :

360.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

JM10-59

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Rat Site: pancreas Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

image 2 :

Application: IF-Tissue Species: Human Site: pancreas Sample: Paraffin-embedded section Antibody concentration: 1/500

image 3 :

Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rat anti-PGP9.5 antibody (HA601515) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601515) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601515

Target name :

PGP9.5

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-Fr,IF-Tissue,IHC-P,WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human PGP95 aa 191-223 / 223.

Uniprot id :

P09936>SwissProt: P09936 Human;SwissProt: Q9R0P9 Mouse;SwissProt: Q00981 Rat

Host :

rabbit

Clone number :

JM10-59

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Human pancreas tissue, rat pancreas tissue, SH-SY5Y cell lysate, A-172 cell lysate, Neuro-2a cell lysate, C6 cell lysate, SH-SY5Y.

Molecular wt :

Predicted band size: 25 kDa

Subcellular location :

Cytoplasm, Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:200 ;IF-Tissue: 1:500 ;IHC-P: 1:2,000 ;WB: 1:10,000 ;IF-Cell: 1:200

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601515_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue with Rat anti-PGP9.5 antibody (HA601515) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601515) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601515_5.jpg

Pic legend5 :

Western blot analysis of PGP9.5 on different lysates with Rat anti-PGP9.5 antibody (HA601515) at 1/10,000 dilution. Lane 1: SH-SY5Y cell lysate Lane 2: A-172 cell lysate Lane 3: LNCaP cell lysate (negative) Lane 4: Neuro-2a cell lysate Lane 5: NIH/3T3 cell lysate (negative) Lane 6: C6 cell lysate Lysates/proteins at 10 µg/Lane. Predicted band size: 25 kDa Observed band size: 25 kDa Exposure time: 10 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601515) at 1/10,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/50,000 dilution was used for 1 hour at room temperature.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601515_6.jpg

Pic legend6 :

Immunocytochemistry analysis of SH-SY5Y cells labeling PGP9.5 with Rat anti-PGP9.5 antibody (HA601515) at 1/200 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-PGP9.5 antibody (HA601515) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/500 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage