Immunocytochemistry analysis of HeLa cells labeling Myc tag with Mouse anti-Myc tag antibody (HA601513) at 1/200 dilution. HeLa cells, transfected with empty control (top, negative) / Myc-tagged Claudin18.2 (C-terminal) (middle, positive) / Myc-tagged Histone H3.1 (N-terminal) (bottom, positive) expression vector, respectively, were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Myc tag antibody (HA601513) at 1/200 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Myc tag was immunoprecipitated from 0.2 mg 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate with HA601513 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601513 at 1/20,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate (input) Lane 2: HA601513 IP in 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate Lane 3: Mouse IgG instead of HA601513 in 293T transfected with Myc-tagged Histone H3.1 (N-terminal) cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 10 seconds; ECL: K1801