Aldolase A/B/C | HUABIO HA601511 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Aldolase A/B/C

catalog :

HA601511

quantity :

100μl

price :

360.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH01-81

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: brain Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse brain tissue with Mouse anti-Aldolase A/B/C antibody (HA601511) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601511) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded rat brain tissue with Mouse anti-Aldolase A/B/C antibody (HA601511) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601511) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601511

Target name :

Aldolase A/B/C

Species reactivity :

Human,Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within Human Aldolase C aa 50-100.

Uniprot id :

P09972>SwissProt: P09972 Human;SwissProt: P05063 Mouse;SwissProt: P09117 Rat

Host :

rabbit

Clone number :

PSH01-81

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse brain tissue, rat brain tissue, U-87 MG cell lysate, RAW264.7 cell lysate, C6 cell lysate, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 40 kDa

Subcellular location :

cytoskeleton, cytosol, extracellular exosome, extracellular region

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:5,000 ;WB: 1:5,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601511_4.jpg

Pic legend4 :

Western blot analysis of Aldolase A/B/C on different lysates with Mouse anti-Aldolase A/B/C antibody (HA601511) at 1/5,000 dilution. Lane 1: U-87 MG cell lysate Lane 2: RAW264.7 cell lysate Lane 3: C6 cell lysate Lane 4: Mouse brain tissue lysate Lane 5: Mouse pancreas tissue lysate (negative) Lane 6: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 40 kDa Observed band size: 40 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601511) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage