CD25 / IL-2 Receptor alpha | HUABIO HA601486 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

CD25 / IL-2 Receptor alpha

catalog :

HA601486

quantity :

100μl

price :

385.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH14-46

reactivity :

human, mouse

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Flow cytometric analysis of human peripheral blood cells labeling CD25 / IL-2 Receptor alpha (HA601486) and CD4 (HA600121F, APC). Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601486, 1/1,000). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Compared with Mouse IgG Isotype Control (iFluor™ 488, 1/1,000).

image 2 :

Immunohistochemical analysis of paraffin-embedded mouse spleen tissue with Mouse anti-CD25 / IL-2 Receptor alpha antibody (HA601486) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601486) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Application: IF-Tissue Species: Mouse Site: spleen Sample: Paraffin-embedded section Antibody concentration: 1/1,000

product information

SKU :

HA601486

Target name :

CD25 / IL-2 Receptor alpha

Species reactivity :

Human,Mouse

Applications :

FC,IHC-P,IF-Tissue

Conjugate :

Non-conjugated

Uniprot id :

P01589>SwissProt: P01589 Human;SwissProt: P01590 Mouse

Host :

Mouse

Clone number :

PSH14-46

Isotype :

IgG1

Size :

100μl

List Price :

385.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Human peripheral blood, mouse spleen tissue.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Membrane

Concentration :

2 mg/mL.

Recommended dilutions :

FC: 1:1,000 (For concentrate), 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood (For predilute) ;IHC-P: 1:1,000 ;IF-Tissue: 1:1,000

more info or order :

HUABIO product webpage