product summary
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company name :
HUABIO
product type :
antibody
product name :
NF-L
catalog :
HA601472
quantity :
100μl
price :
360.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
PS02-10
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
HUABIO product webpage
image
image 1 :
HUABIO HA601472 image 1
Application: IHC-Fr Species: Mouse Site: cerebellum Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required
image 2 :
HUABIO HA601472 image 2
Western blot analysis of NF-L on different lysates with Chicken anti-NF-L antibody (HA601472) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (20 µg/Lane) Lane 2: Mouse lung tissue lysate (negative) (20 µg/Lane) Lane 3: Rat brain tissue lysate (20 µg/Lane) Lane 4: Rat lung tissue lysate (negative) (20 µg/Lane) Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 1 minute 50 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601472) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit Anti-Chicken IgY - HRP Secondary Antibody (HA1026) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA601472
Target name :
NF-L
Species reactivity :
Mouse,Rat
Applications :
IHC-Fr,WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein.
Uniprot id :
P08551>SwissProt: P08551 Mouse;SwissProt: P19527 Rat
Host :
rabbit
Clone number :
PS02-10
Isotype :
IgY
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
Mouse brain tissue lysate, Rat brain tissue lysate.
Molecular wt :
Predicted band size: 62 kDa
Subcellular location :
Cell projection, axon, Cytoplasm, cytoskeleton.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-Fr: 1:200 ;WB: 1:2,000
Advanced Validation :
Relative expression (RE)
more info or order :
HUABIO product webpage
company information
HUABIO
Boston, Massachusetts
support@huabio.com
https://www.huabio.com
1-857-353-6600
headquarters: USA
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!