VGLUT1 | HUABIO HA601450 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

VGLUT1

catalog :

HA601450

quantity :

100μl

price :

360.00 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-53

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Rat Site: hippocampus Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-VGLUT1 antibody (HA601450) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601450) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601450

Target name :

VGLUT1

Species reactivity :

Mouse,Rat

Applications :

IHC-Fr,IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Mouse VGLUT1 aa 1-560.

Uniprot id :

Q3TXX4>SwissProt: Q3TXX4 Mouse;SwissProt: Q62634 Rat

Host :

rabbit

Clone number :

PSH10-53

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse cerebellum tissue, rat cerebellum tissue, Mouse brain tissue lysate.

Molecular wt :

Predicted band size: 62 kDa

Subcellular location :

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane, Cell membrane, Synapse, synaptosome.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:2,000 ;WB: 1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601450_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-VGLUT1 antibody (HA601450) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601450) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601450_5.jpg

Pic legend5 :

Western blot analysis of VGLUT1 on different lysates with Mouse anti-VGLUT1 antibody (HA601450) at 1/5,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Mouse lung tissue lysate (no heat) (negative) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 62 kDa Observed band size: 62 kDa Exposure time: 42 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601450) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage