MTAP | HUABIO HA601445 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MTAP

catalog :

HA601445

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH13-98

reactivity :

human, mouse

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MTAP on different lysates with Mouse anti-MTAP antibody (HA601445) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate Lane 2: HeLa cell lysate Lane 3: A549 cell lysate (negative) Lane 4: NIH/3T3 cell lysate Lane 5: C2C12 cell lysate Lane 6: C6 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 31 kDa Observed band size: 31 kDa Exposure time: 1 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601445) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of NIH/3T3 cells labeling MTAP with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of C6 cells labeling MTAP with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-MTAP antibody (HA601445) at 1/50 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA601445

Target name :

MTAP

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Uniprot id :

Q13126>SwissProt: Q13126 Human;SwissProt: Q9CQ65 Mouse;Entrez Gene: 298227 Rat

Host :

Mouse

Clone number :

PSH13-98

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HCT 116 cell lysate, HeLa cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, C6 cell lysate, NIH/3T3, C6.

Molecular wt :

Predicted band size: 31 kDa

Subcellular location :

Cytoplasm, Nucleus.

Concentration :

0.375 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IF-Cell: 1:50

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage