product summary
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company name :
HUABIO
product type :
antibody
product name :
NF-M
catalog :
HA601444
quantity :
100μl
price :
360.00 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM11-20
reactivity :
mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
image
image 1 :

Application: IHC-Fr
Species: Mouse
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/500 (NF-M, HA601444, Guinea pig, red); 1/500 (QKI-6, HA723588, Rabbit, green)
Antigen retrieval: Not required
image 2 :

Application: IHC-Fr
Species: Rat
Site: cerebellum
Sample: Frozen section
Antibody concentration: 1/500 (NF-M, HA601444, Guinea pig, red); 1/500 (QKI-6, HA723588, Rabbit, green)
Antigen retrieval: Not required
image 3 :

Western blot analysis of NF-M on different lysates with Guinea pig anti-NF-M antibody (HA601444) at 1/5,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Mouse liver tissue lysate (negative)
Lane 3: Rat brain tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 102 kDa
Observed band size: 140 kDa
Exposure time: 8 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601444) at 1/5,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Rabbit anti-Guinea pig IgG H&L - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA601444
Target name :
NF-M
Species reactivity :
Mouse,Rat
Applications :
IHC-Fr,WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within Human NEFM aa 651-692 / 916.
Uniprot id :
P08553>SwissProt: P08553 Mouse;SwissProt: P12839 Rat
Host :
rabbit
Clone number :
JM11-20
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
Mouse cerebellum tissue, Rat cerebellum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.
Molecular wt :
Predicted band size: 102 kDa
Subcellular location :
Cell projection, Cytoplasm, Cytoskeleton, Intermediate filament.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-Fr: 1:500
;WB: 1:5,000
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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