p75 NGF Receptor | HUABIO HA601436 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

p75 NGF Receptor

catalog :

HA601436

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

PSH13-26

reactivity :

mouse, rat

application :

western blot, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: brain (medial septum) Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: colon Sample: Frozen section Antibody concentration: 1/200 Antigen retrieval: Not required

image 3 :

Western blot analysis of p75 NGF Receptor on different lysates with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate Lane 2: Rat brain tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 46 kDa Observed band size: 70 kDa Exposure time: 59 seconds; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601436) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA601436

Target name :

p75 NGF Receptor

Species reactivity :

Mouse,Rat

Applications :

IHC-Fr,WB,IF-Cell

Conjugate :

Non-conjugated

Uniprot id :

Q9Z0W1>SwissProt: Q9Z0W1 Mouse;SwissProt: P07174 Rat

Host :

Rat

Clone number :

PSH13-26

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Rat Monoclonal Antibody

Positive control :

Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 46 kDa

Subcellular location :

Cell membrane, Cytoplasm, Perikaryon, Cell projection, growth cone, dendritic spine.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:200 ;WB: 1:2,000 ;IF-Cell: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601436_4.jpg

Pic legend4 :

Immunocytochemistry analysis of Neuro-2a cells labeling p75 NGF Receptor with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-p75 NGF Receptor antibody (HA601436) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

more info or order :

HUABIO product webpage