HLA Class I | HUABIO HA601424 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

HLA Class I

catalog :

HA601424

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH12-15

reactivity :

human

application :

flow cytometry

more info or order :

HUABIO product webpage

image

image 1 :

Immunocytochemistry analysis of Jurkat cells labeling HLA Class I with Mouse anti-HLA Class I antibody (HA601424) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-HLA Class I antibody (HA601424) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 2 :

Flow cytometric analysis of K-562 (left, negative) and Jurkat (right, positive) cells labeling HLA Class I. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601424, 1/1,000) (red) compared with Mouse IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

product information

SKU :

HA601424

Target name :

HLA Class I

Species reactivity :

Human

Applications :

IF-Cell,FC

Conjugate :

Non-conjugated

Uniprot id :

P04439>SwissProt: P04439 Human;SwissProt: P01889 Human;SwissProt: P10321 Human

Host :

Mouse

Clone number :

PSH12-15

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Jurkat.

Molecular wt :

Predicted band size: 41 kDa

Subcellular location :

Cell membrane, Endoplasmic reticulum membrane.

Concentration :

1 mg/mL.

Recommended dilutions :

IF-Cell: 1:100 ;FC: 1:1,000

Advanced Validation :

Relative expression (RE)

more info or order :

HUABIO product webpage