MAG | HUABIO HA601414 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MAG

catalog :

HA601414

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH02-41

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1/500 Antigen retrieval: Not required

image 2 :

Application: IF-tissue Species: Mouse Site: Cerebellum Sample: Paraffin-embedded section Antibody concentration: 1/500

image 3 :

Immunohistochemical analysis of paraffin-embedded human cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601414

Target name :

MAG

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

IHC-Fr,IHC-P,WB,IF-Tissue

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MAG aa 1-536 / 626.

Uniprot id :

P20916>SwissProt: P20916 Human;SwissProt: P20917 Mouse;SwissProt: P07722 Rat

Host :

rabbit

Clone number :

PSH02-41

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Human cerebellum tissue, mouse cerebellum tissue, rat cerebellum tissue, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate.

Molecular wt :

Predicted band size: 69 kDa

Subcellular location :

Cell membrane, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500-1:1,000 ;IHC-P: 1:500 ;WB: 1:2,000 ;IF-Tissue: 1:500-1:1,000

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601414_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601414_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Rat anti-MAG antibody (HA601414) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601414) at 1/500dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601414_6.jpg

Pic legend6 :

Western blot analysis of MAG on different lysates with Rat anti-MAG antibody (HA601414) at 1/2,000 dilution. Lane 1: Mouse cerebellum tissue lysate Lane 2: Mouse liver tissue lysate (negative) Lane 3: Rat cerebellum tissue lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 100 kDa Exposure time: Lane 1-2: 13 seconds; Lane 3: 21 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601414) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage