Dopamine Transporter | HUABIO HA601404 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Dopamine Transporter

catalog :

HA601404

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH10-49

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Application: IHC-Fr Species: Mouse Site: Striatum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: The section was pre-treated using 1% SDS buffer (in PBS, pH 7.4) for 5 minutes at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse striatum tissue with Rat anti-Dopamine Transporter antibody (HA601404) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601404) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601404

Target name :

Dopamine Transporter

Species reactivity :

Mouse,Rat,Human,Cynomolgus monkey,Pig

Applications :

IHC-Fr,IHC-P,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein.

Uniprot id :

Q61327>SwissProt: Q61327 Mouse;SwissProt: P23977 Rat

Host :

rabbit

Clone number :

PSH10-49

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse striatum tissue, rat striatum tissue, Mouse brain tissue lysate, Rat brain tissue lysate.

Molecular wt :

Predicted band size: 69 kDa.

Subcellular location :

Cell membrane

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:500 ;IHC-P: 1:500-1:2,000 ;WB: 1:2,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601404_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat striatum tissue with Rat anti-Dopamine Transporter antibody (HA601404) at 1/500 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601404) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601404_5.jpg

Pic legend5 :

Western blot analysis of Dopamine Transporter on different lysates with Rat anti-Dopamine Transporter antibody (HA601404) at 1/2,000 dilution. Lane 1: Mouse brain tissue lysate (no heat) Lane 2: Rat brain tissue lysate (no heat) Notice: no heat means the lysate is not boiled. Lysates/proteins at 20 µg/Lane. Predicted band size: 69 kDa Observed band size: 75 kDa Exposure time: 4 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601404) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Rat IgG H&L - HRP Secondary Antibody (HA1023) at 1/5,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage