QKI-5 | HUABIO HA601378 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

QKI-5

catalog :

HA601378

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH10-22

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebellum Sample: Frozen section Antibody concentration: 1:500 Antigen retrieval: Not required

image 2 :

Western blot analysis of QKI-5 on different lysates with Mouse anti-QKI-5 antibody (HA601378) at 1/2,000 dilution. Lane 1: K-562 cell lysate Lane 2: HeLa cell lysate Lane 3: Mouse brain tissue lysate Lane 4: Mouse cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 38 kDa Observed band size: 38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601378) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/20,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/20,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601378

Target name :

QKI-5

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

WB,IHC-P,IHC-Fr,IP

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human QKI-5 aa 301-341 / 341.

Uniprot id :

Q96PU8-1>SwissProt: Q96PU8-1 Human;SwissProt: Q9QYS9 Mouse;SwissProt: Q91XU1 Rat

Host :

Mouse

Clone number :

PSH10-22

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

K-562 cell lysate, HeLa cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, mouse cerebellum tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 38 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:5,000-1:20,000 ;IHC-Fr: 1:500 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601378_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI-5 antibody (HA601378) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601378) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601378_5.jpg

Pic legend5 :

Indirect ELISA analysis of QKI-5 on different peptides.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601378_6.jpg

Pic legend6 :

QKI-5 was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601378 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601378 at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601378 IP in HeLa cell lysate Lane 3: Mouse IgG instead of HA601378 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 1 minute 7 seconds; ECL: K1801

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA601378_7.jpg

Pic legend7 :

more info or order :

HUABIO product webpage