QKI | HUABIO HA601377 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

QKI

catalog :

HA601377

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH10-21

reactivity :

human, mouse, rat

application :

western blot, immunoprecipitation, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of QKI on different lysates with Mouse anti-QKI antibody (HA601377) at 1/2,000 dilution. Lane 1: HeLa cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Rat cerebellum tissue lysate Lysates/proteins at 30 µg/Lane. Predicted band size: 38 kDa Observed band size: 35-38 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601377) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunocytochemistry analysis of HeLa cells labeling QKI with Mouse anti-QKI antibody (HA601377) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-QKI antibody (HA601377) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunofluorescence analysis of frozen mouse cerebellum tissue with Mouse anti-QKI antibody (HA601377) at 1/500 dilution. The section was not undergone antigen retrieval. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601377, green) at 1/500 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).

product information

SKU :

HA601377

Target name :

QKI

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IF-Cell,IHC-P,IHC-Fr,IP

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human QKI aa 1-341 / 341.

Uniprot id :

Q96PU8>SwissProt: Q96PU8 Human;SwissProt: Q9QYS9 Mouse;SwissProt: Q91XU1 Rat

Host :

Mouse

Clone number :

PSH10-21

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, Mouse brain tissue lysate, Mouse cerebellum tissue lysate, Rat cerebellum tissue lysate, HeLa, mouse cerebellum tissue, rat cerebellum tissue.

Molecular wt :

Predicted band size: 38 kDa

Subcellular location :

Nucleus, Cytoplasm.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IF-Cell: 1:100 ;IHC-P: 1:200 ;IHC-Fr: 1:500 ;IP: 1-2μg/sample

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601377_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue with Mouse anti-QKI antibody (HA601377) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601377) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601377_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue with Mouse anti-QKI antibody (HA601377) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601377) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601377_6.jpg

Pic legend6 :

QKI was immunoprecipitated from 0.2 mg HeLa cell lysate with HA601377 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA601377 at 1/1,000 dilution. Anti-Mouse IgG for IP Nano-secondary antibody (NBI02H) at 1/5,000 dilution was used for 1 hour at room temperature. Lane 1: HeLa cell lysate (input) Lane 2: HA601377 IP in HeLa cell lysate Lane 3: Mouse IgG instead of HA601377 in HeLa cell lysate Blocking/Dilution buffer: 5% NFDM/TBST Exposure time: 3 minutes; ECL: K1801

more info or order :

HUABIO product webpage