Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA601374, Guinea pig, Red); 1/1,000 (Iba1, ET1705-78, Rabbit, Green) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

Western blot analysis of c-Fos on different lysates with Guinea pig anti-c-Fos antibody (HA601374) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41-55 kDa Exposure time: 6 seconds; ECL: K1801; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601374) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/10,000 dilution was used for 1 hour at room temperature.