c-Fos | HUABIO HA601373 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

c-Fos

catalog :

HA601373

quantity :

100μl

price :

330 USD

clonality :

polyclonal

host :

domestic rabbit

conjugate :

nonconjugated

clone name :

PSH05-77

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section, immunohistochemistry - frozen section

more info or order :

HUABIO product webpage

image

image 1 :

Application: IHC-Fr Species: Mouse Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/5,000 (c-Fos, HA601373, Rat, green); 1/1,000 (Iba1, ET1705-78, Rabbit, red) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

image 2 :

Application: IHC-Fr Species: Rat Site: Cerebral cortex (restraint stress induced) Sample: Frozen section Antibody concentration: 1/2,000 (c-Fos, HA601373, Rat, green); 1/1,000 (Iba1, ET1705-78, Rabbit, red) Antigen retrieval: Not required Important Notice: Blocking buffer and antibody dilution buffer are recommended to use TBS buffer instead of PBS buffer.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse hippocampus (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA601373) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601373) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601373

Target name :

c-Fos

Species reactivity :

Human,Mouse,Rat,Cynomolgus monkey,Pig

Applications :

IHC-Fr,IHC-P,IF-Tissue,IF-Cell,WB

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human Protein c-Fos aa 1-380.

Uniprot id :

P01100>SwissProt: P01100 Human;SwissProt: P01101 Mouse;SwissProt: P12841 Rat

Host :

rabbit

Clone number :

PSH05-77

Isotype :

IgG1

Size :

100μl

List Price :

330 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Chimeric Antibody Antibody

Positive control :

Mouse brain tissue, rat brain tissue.

Subcellular location :

Nucleus, Endoplasmic reticulum, Cytoplasm, cytosol.

Concentration :

1 mg/mL.

Recommended dilutions :

IHC-Fr: 1:2,000-1:5,000 ;IHC-P: 1:5,000 ;IF-Tissue: 1:1,000-1:2,000 ;IF-Cell: 1:1,000 ;WB: 1:5,000-1:10,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601373_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA601373) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601373) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601373_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat brain (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA601373) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601373) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601373_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat hippocampus (restraint stress induced) tissue with Rabbit anti-c-Fos antibody (HA601373) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601373) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img7 :

https://storage.huabio.cn/huabio/productImg/HA601373_7.jpg

Pic legend7 :

Immunocytochemistry analysis of HeLa cells untreated / HeLa cells starved 16 hours then treated with 200nM TPA for 4 hours labeling c-Fos with Rat anti-c-Fos antibody (HA601373) at 1/1,000 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rat anti-c-Fos antibody (HA601373) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rat IgG H&L (iFluor™ 488, HA1133) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

Pic img8 :

https://storage.huabio.cn/huabio/productImg/HA601373_8.jpg

Pic legend8 :

Western blot analysis of c-Fos on different lysates with Rat anti-c-Fos antibody (HA601373) at 1/5,000 dilution. Lane 1: HeLa cell lysate Lane 2: HeLa serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lane 3: RAW264.7 cell lysate Lane 4: RAW264.7 serum starved for 16 hours then add 200 nM TPA for 4 hours cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 41 kDa Observed band size: 41-55 kDa Exposure time: 8 seconds; ECL: K1801; Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601373) at 1/5,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rat IgG - HRP Secondary Antibody (HA1023) at 1/10,000 dilution was used for 1 hour at room temperature.

more info or order :

HUABIO product webpage