product summary
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company name :
HUABIO
product type :
antibody
product name :
Iba1
catalog :
HA601368
quantity :
100μl
price :
330 USD
clonality :
polyclonal
host :
domestic rabbit
conjugate :
nonconjugated
clone name :
JM36-62
reactivity :
human, mouse, rat
application :
western blot, immunohistochemistry - frozen section
more info or order :
image
image 1 :

Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required
image 2 :

Application: IHC-Fr
Species: Mouse
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500 (Iba1, HA601368, red); 1/500 (NeuN, HA601111, green)
Antigen retrieval: Not required
image 3 :

Application: IHC-Fr
Species: Mouse
Site: Hippocampus
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required
product information
SKU :
HA601368
Target name :
Iba1
Species reactivity :
Human,Mouse,Rat,Cynomolgus monkey,Pig
Applications :
IHC-Fr,WB
Conjugate :
Non-conjugated
Immunogen :
Synthetic peptide within N-terminal human Iba1.
Uniprot id :
P55008>SwissProt: P55008 Human;SwissProt: O70200 Mouse;SwissProt: P55009 Rat
Host :
rabbit
Clone number :
JM36-62
Size :
100μl
List Price :
330 USD
Storage Buffer :
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Chimeric Antibody Antibody
Positive control :
Mouse brain tissue, mouse hippocampus tissue, rat brain tissue, THP-1 cell lysates.
Molecular wt :
Predicted band size: 17 kDa
Subcellular location :
Cytoplasm, cytoskeleton, Cell projection, ruffle membrane, Cell projection, phagocytic cup.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-Fr: 1:200-1:500
;WB: 1:1,000
Pic img4 :
https://storage.huabio.cn/huabio/productImg/HA601368_4.jpg
Pic legend4 :
Application: IHC-Fr
Species: Rat
Site: Cerebral cortex
Sample: Frozen section
Antibody concentration: 1/500
Antigen retrieval: Not required
Pic img5 :
https://storage.huabio.cn/huabio/productImg/HA601368_5.jpg
Pic legend5 :
Western blot analysis of Iba1 on THP-1 cell lysates with Guinea pig anti-Iba1 antibody (HA601368) at 1/1,000 dilution.
Lysates/proteins at 10 µg/Lane.
Predicted band size: 17 kDa
Observed band size: 12 kDa
Exposure time: 2 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601368) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Rabbit anti-Guinea pig IgG - HRP Secondary Antibody (HA1021) at 1/5,000 dilution was used for 1 hour at room temperature.
more info or order :
company information

HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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