COMP | HUABIO HA601354 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

COMP

catalog :

HA601354

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A10H2

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution. Lane 1: Mouse articular cartilage tissue lysate (40 µg/Lane) Lane 2: Mouse liver tissue lysate (negative) (40 µg/Lane) Lane 3: Rat articular cartilage tissue lysate (40 µg/Lane) Lane 4: Rat liver tissue lysate (negative) (40 µg/Lane) Predicted band size: 83 kDa Observed band size: 100 kDa Exposure time: 3 minutes; ECL: K1802; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of COMP on different lysates with Mouse anti-COMP antibody (HA601354) at 1/1,000 dilution. Lane 1: HCT 116 cell lysate (15 µg/Lane) Lane 2: HCT 116 cell lysate (30 µg/Lane) Predicted band size: 83 kDa Observed band size: 100 kDa Exposure time: 3 minute; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601354) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded mouse cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601354

Target name :

COMP

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Synthetic peptide within human COMP aa 321-370.

Uniprot id :

P49747>SwissProt: P49747 Human;SwissProt: Q9R0G6 Mouse;SwissProt: P35444 Rat

Host :

Mouse

Clone number :

A10H2

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

Mouse articular cartilage tissue lysate, Rat articular cartilage tissue lysate, HCT 116 cell lysate, mouse cartilage tissue, rat cartilage tissue.

Molecular wt :

Predicted band size: 83 kDa

Subcellular location :

Secreted, extracellular space, extracellular matrix.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:400

Advanced Validation :

Relative expression (RE)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601354_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded mouse liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601354_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded rat cartilage tissue with Mouse anti-COMP antibody (HA601354) at 1/400 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601354_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat liver tissue (negative) with Mouse anti-COMP antibody (HA601354) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) in water bath overnight. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601354) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage