Rbm15 | HUABIO HA601347 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Rbm15

catalog :

HA601347

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A10H4

reactivity :

green monkey, human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Rbm15 on different lysates with Mouse anti-Rbm15 antibody (HA601347) at 1/1,000 dilution. Lane 1: HeLa cell lysate Lane 2: 293T cell lysate Lane 3: 22RV1 cell lysate Lane 4: C6 cell lysate Lane 5: PC-12 cell lysate Lane 6: COS-1 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601347) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of Rbm15 on different lysates with Mouse anti-Rbm15 antibody (HA601347) at 1/1,000 dilution. Lane 1: C2C12 cell lysate Lane 2: RAW264.7 cell lysate Lysates/proteins at 15 µg/Lane. Predicted band size: 107 kDa Observed band size: 107 kDa Exposure time: 25 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601347) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601347

Target name :

Rbm15

Species reactivity :

Human,Mouse,Rat,Monkey

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human RBM15 aa 450-850.

Uniprot id :

Q96T37>SwissProt: Q96T37 Human;SwissProt: Q6PHZ5 Mouse;SwissProt: M0R3Z8 Rat;SwissProt: A0A0D9S6H4 Green monkey

Host :

Mouse

Clone number :

A10H4

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, 293T cell lysate, 22RV1 cell lysate, C6 cell lysate, PC-12 cell lysate, COS-1 cell lysate, C2C12 cell lysate, RAW264.7 cell lysate, human breast cancer tissue, human liver cancer tissue, mouse lymph nodes tissue, rat lymph nodes tissue.

Molecular wt :

Predicted band size: 107 kDa

Subcellular location :

Nucleus.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:100

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601347_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601347_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded mouse lymph nodes tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img6 :

https://storage.huabio.cn/huabio/productImg/HA601347_6.jpg

Pic legend6 :

Immunohistochemical analysis of paraffin-embedded rat lymph nodes tissue with Mouse anti-Rbm15 antibody (HA601347) at 1/100 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601347) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage