Human CD45RA | HUABIO HA601341 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human CD45RA

catalog :

HA601341

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

PSH04-97

reactivity :

human

application :

flow cytometry, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Flow cytometric analysis of human peripheral blood lymphocytes labeling Human CD45RA. Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA601341, 1μg/mL) (red). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Mouse IgG Secondary antibody (HA1125) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; blue).

image 2 :

Immunocytochemistry analysis of Jurkat cells labeling Human CD45RA with Mouse anti-Human CD45RA antibody (HA601341) at 1/100 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-Human CD45RA antibody (HA601341) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. beta Tubulin (ET1602-4, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Rabbit IgG H&L (iFluor™ 594, HA1122) were used as the secondary antibody at 1/1,000 dilution.

image 3 :

Immunocytochemistry analysis of Raji cells labeling Human CD45RA with Rabbit anti-Human CD45RA antibody (HA601341) at 1/500 dilution. Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Human CD45RA antibody (HA601341) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

product information

SKU :

HA601341

Target name :

Human CD45RA

Species reactivity :

Human

Applications :

FC,IF-Cell,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within

Uniprot id :

P08575>SwissProt: P08575 Human

Host :

Mouse

Clone number :

PSH04-97

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

human peripheral blood lymphocytes, Jurkat, Raji, human lymph nodes tissue, human tonsils tissue.

Molecular wt :

Predicted band size: 147 kDa

Subcellular location :

Cell membrane, Membrane raft.

Concentration :

1 mg/mL.

Recommended dilutions :

FC: 1:1,000 ;IF-Cell: 1:100-1:500 ;IHC-P: 1:5,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601341_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human lymph nodes tissue with Mouse anti-Human CD45RA antibody (HA601341) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601341) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Pic img5 :

https://storage.huabio.cn/huabio/productImg/HA601341_5.jpg

Pic legend5 :

Immunohistochemical analysis of paraffin-embedded human tonsils tissue with Mouse anti-Human CD45RA antibody (HA601341) at 1/5,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601341) at 1/5,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage