Human IgG heavy chain | HUABIO HA601283 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

Human IgG heavy chain

catalog :

HA601283

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

54-76-R

application :

western blot, ELISA, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of Human IgG heavy chain on Recombinant Human IgG protein with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/1,000 dilution. Lysates/proteins at 50 ng/Lane. Observed band size: 50 kDa Exposure time: 15 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601283) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-Human IgG heavy chain antibody (HA601283) at 1/200 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601283) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601283

Target name :

Human IgG heavy chain

Species reactivity :

Human

Applications :

WB,ELISA,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human IgG heavy chain.

Uniprot id :

P01857>SwissProt: P01857 Human;SwissProt: P01859 Human;SwissProt: P01860 Human;SwissProt: P01861 Human

Host :

Mouse

Clone number :

54-76-R

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4).

Form :

Liquid

Storage Instruction :

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

Recombinant Human IgG protein, human tonsil tissue.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000 ;ELISA: 1:1,000-1:20,000 ;IHC-P: 1:200

more info or order :

HUABIO product webpage