product summary
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company name :
HUABIO
product type :
antibody
product name :
Glucosidase 2 subunit beta
catalog :
HA601276
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A6H9-R
reactivity :
human
application :
western blot
more info or order :
image
image 1 :
Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA601276) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Daudi cell lysate
Lane 4: Jurkat cell lysate
Lane 5: A431 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 80 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601276) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
image 2 :
Western blot analysis of Glucosidase 2 subunit beta on different lysates with Mouse anti-Glucosidase 2 subunit beta antibody (HA601276) at 1/2,000 dilution.
Lane 1: HAP1-parental cell lysate
Lane 2: HAP1-Glucosidase 2 subunit beta KD cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 59 kDa
Observed band size: 75 kDa
Exposure time: 15 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601276) at 1/2,000 dilution was used in primary antibody dilution (K1803) at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.
product information
SKU :
HA601276
Target name :
Glucosidase 2 subunit beta
Species reactivity :
Human
Applications :
WB
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within human Glucosidase 2 subunit beta aa 51-250.
Uniprot id :
P14314>SwissProt: P14314 Human
Host :
Mouse
Clone number :
A6H9-R
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
HeLa cell lysate, 293T cell lysate, Daudi cell lysate, Jurkat cell lysate, A431 cell lysate.
Molecular wt :
Predicted band size: 59 kDa
Subcellular location :
Endoplasmic reticulum
Concentration :
1 mg/mL.
Recommended dilutions :
WB: 1:1,000
Advanced Validation :
Knockdown (KD)
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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