alpha Actinin | HUABIO HA601194 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

alpha Actinin

catalog :

HA601194

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A1G2-R

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (HA601194) at 1/1,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: NIH/3T3 cell lysate (20 µg/Lane) Lane 4: C6 cell lysate (20 µg/Lane) Lane 5: Mouse heart tissue lysate (40 µg/Lane) Lane 6: Rat colon tissue lysate (40 µg/Lane) Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 8 seconds; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601194) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of alpha Actinin on different lysates with Mouse anti-alpha Actinin antibody (HA601194) at 1/5,000 dilution. Lane 1: HeLa cell lysate (20 µg/Lane) Lane 2: A431 cell lysate (20 µg/Lane) Lane 3: A549 cell lysate (20 µg/Lane) Lane 4: HepG2 cell lysate (20 µg/Lane) Lane 5: MCF7 cell lysate (20 µg/Lane) Lane 6: NIH/3T3 cell lysate (20 µg/Lane) Lane 7: PC-12 cell lysate (20 µg/Lane) Lane 8: C6 cell lysate (20 µg/Lane) Predicted band size: 103 kDa Observed band size: 103 kDa Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601194) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 3 :

Immunohistochemical analysis of paraffin-embedded human breast tissue with Mouse anti-alpha Actinin antibody (HA601194) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601194) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601194

Target name :

alpha Actinin

Species reactivity :

Human,Mouse,Rat

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within Human ACTN1 aa 388-619 / 892.

Uniprot id :

P12814>SwissProt: P12814 Human;SwissProt: Q7TPR4 Mouse;SwissProt: Q9Z1P2 Rat

Host :

Mouse

Clone number :

A1G2-R

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

HeLa cell lysate, A431 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, Mouse heart tissue lysate, Rat colon tissue lysate, A549 cell lysate, HepG2 cell lysate, MCF7 cell lysate, PC-12 cell lysate, human breast tissue, human breast carcinoma tissue.

Molecular wt :

Predicted band size: 103 kDa

Subcellular location :

Cell membrane, cytoskeleton, Z line, cell junction, ruffle.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:1,000-1:5,000 ;IHC-P: 1:6,000

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601194_4.jpg

Pic legend4 :

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue with Mouse anti-alpha Actinin antibody (HA601194) at 1/6,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601194) at 1/6,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

more info or order :

HUABIO product webpage