mCherry | HUABIO HA601186 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

mCherry

catalog :

HA601186

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A9F2

application :

western blot

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of mCherry on different lysates with Mouse anti-mCherry antibody (HA601186) at 1/2,000 dilution. Lane 1: 293T-WT cell lysate (5 µg/Lane) Lane 2: 293T-OE-mCherry cell lysate (5 µg/Lane) Lane 3: 293T-OE-mCherry cell lysate (2.5 µg/Lane) Lane 4: 293T-OE-mCherry cell lysate (1.25 µg/Lane) Lane 5: 293T-OE-mCherry cell lysate (0.625 µg/Lane) Predicted band size: 27 kDa Observed band size: 27 kDa Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601186) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/150,000 dilution was used for 1 hour at room temperature.

image 2 :

Western blot analysis of mCherry on mCherry recombinant protein with Mouse anti-mCherry antibody (HA601186) at 1/1,000 dilution. Lane 1: mCherry recombinant protein, 500ng/Lane Lane 2: mCherry recombinant protein, 250ng/Lane Lane 3: mCherry recombinant protein, 125ng/Lane Lane 4: mCherry recombinant protein, 62.5ng/Lane Exposure time: 30 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601186) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/150,000 dilution was used for 1 hour at room temperature.

image 3 :

Western blot analysis of mCherry on different lysates with Mouse anti-mCherry antibody (HA601186) at 1/5,000 dilution. Lane 1: 293T cell lysate Lane 2: 293T transfected with mCherry-tagged cell lysate Lane 3: 293T transfected with GFP-tagged cell lysate (negative) Lysates/proteins at 5 µg/Lane. Exposure time: 3 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601186) at 1/5,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

product information

SKU :

HA601186

Target name :

mCherry

Species reactivity :

Species independent

Applications :

WB,IF-Cell

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within mCherry aa 1-236 / 236.

Uniprot id :

X5DSL3>SwissProt: X5DSL3 AnaplasmaMarginale

Host :

Mouse

Clone number :

A9F2

Isotype :

IgG2b

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Concentration :

1.17 mg/mL.

Recommended dilutions :

WB: 1:2,000-1:5,000 ;IF-Cell: 1:2,000

Advanced Validation :

Cell treatment (CT)

Pic img4 :

https://storage.huabio.cn/huabio/productImg/HA601186_4.jpg

Pic legend4 :

Immunocytochemistry analysis of 293T transfected with or without mCherry cells labeling mCherry with Mouse anti-mCherry antibody (HA601186) at 1/2,000 dilution. Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Mouse anti-mCherry antibody (HA601186) at 1/2,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. As expected, green antibody staining is only visible in cells expressing mCherry, and green and red signals can be superimposed.

more info or order :

HUABIO product webpage