MLH1 | HUABIO HA601184 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

MLH1

catalog :

HA601184

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

A10A3

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of MLH1 on different lysates with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution. Lane 1: A431 cell lysate Lane 2: HeLa cell lysate Lane 3: Daudi cell lysate Lane 4: K-562 cell lysate Lane 5: HL-60 cell lysate Lane 6: SW480 cell lysate Lysates/proteins at 20 µg/Lane. Predicted band size: 84.6 kDa Observed band size: 85 kDa Exposure time: 1 minutes 40 seconds; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601184) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/50,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human appendix tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

image 3 :

Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Mouse anti-MLH1 antibody (HA601184) at 1/1,000 dilution. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601184) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601184

Target name :

MLH1

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human MLH1 aa 301-600 / 756.

Uniprot id :

P40692>SwissProt: P40692 Human

Host :

Mouse

Clone number :

A10A3

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Mouse monoclonal Antibody

Positive control :

A431 cell lysate, HeLa cell lysate, Daudi cell lysate, K-562 cell lysate, HL-60 cell lysate, SW480 cell lysate, human appendix tissue, human tonsil tissue.

Molecular wt :

Predicted band size: 85 kDa

Subcellular location :

Nucleus, Chromosome.

Concentration :

1mg/mL

Recommended dilutions :

WB: 1:1,000 ;IHC-P: 1:1,000

more info or order :

HUABIO product webpage