product summary
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company name :
HUABIO
product type :
antibody
product name :
ddx24
catalog :
HA601183
quantity :
100μl
price :
360.00 USD
clonality :
monoclonal
host :
mouse
conjugate :
nonconjugated
clone name :
A3B10-R
application :
immunohistochemistry - paraffin section
more info or order :
image
image 1 :
Immunofluorescence analysis of paraffin-embedded zebrafish (head) tissue labeling ddx24 with Mouse anti-ddx24 antibody (HA601183) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601183, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 2 :
Immunofluorescence analysis of paraffin-embedded zebrafish (tail) tissue labeling ddx24 with Mouse anti-ddx24 antibody (HA601183) at 1/50 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 10% negative goat serum for 1 hour at room temperature, washed with PBS, and then probed with the primary antibody (HA601183, green) at 1/50 dilution overnight at 4 ℃, washed with PBS. Goat Anti-Mouse IgG H&L (iFluor™ 488, HA1125) was used as the secondary antibody at 1/1,000 dilution. Nuclei were counterstained with DAPI (blue).
image 3 :
Immunohistochemical analysis of paraffin-embedded zebrafish tissue with Mouse anti-ddx24 antibody (HA601183) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601183) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
product information
SKU :
HA601183
Target name :
ddx24
Species reactivity :
zebrafish
Applications :
IHC-P,IF-Tissue
Conjugate :
Non-conjugated
Immunogen :
Recombinant protein within zebrafish ddx24 aa 562-819 / 832.
Uniprot id :
A0A0R4IA19>SwissProt: A0A0R4IA19 Zebrafish
Host :
Mouse
Clone number :
A3B10-R
Isotype :
IgG1
Size :
100μl
List Price :
360.00 USD
Storage Buffer :
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Form :
Liquid
Storage Instruction :
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Purity :
Protein A affinity purified.
Product type :
Recombinant Mouse monoclonal Antibody
Positive control :
Zebrafish tissue.
Molecular wt :
Predicted band size: 94.5 kDa
Subcellular location :
Nucleolus.
Concentration :
1 mg/mL.
Recommended dilutions :
IHC-P: 1:1,000
;IF-Tissue: 1:50
more info or order :
company information
HUABIO
Founded in 2007, HUABIO is dedicated to developing high-quality antibodies that advance innovation. We are passionate about the accuracy, efficiency, and consistency of our products. That is why we have invested in new production platforms, like recombinant rabbit monoclonals, alpaca nanobodies, and adopted aggressive QA standards to deliver cutting-edge antibodies with uncompromised quality.
We hope to see you at your next discovery!
We hope to see you at your next discovery!
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