DAP Kinase 1 | HUABIO HA601162 product information

product summary

company name :

HUABIO

product type :

antibody

product name :

DAP Kinase 1

catalog :

HA601162

quantity :

100μl

price :

360.00 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

12B2-R

reactivity :

human

application :

western blot, immunohistochemistry - paraffin section

more info or order :

HUABIO product webpage

image

image 1 :

Western blot analysis of DAP Kinase 1 on different lysates with Mouse anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution. Lane 1: A549 cell lysate (30 µg/Lane) Lane 2: TF-1 cell lysate (30 µg/Lane) Lane 3: HepG2 cell lysate (30 µg/Lane) Lane 4: Human brain tissue lysate (30 µg/Lane) Predicted band size: 160 kDa Observed band size: 160 kDa Exposure time: 2 minutes; ECL: K1801; 4-20% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA601162) at 1/2,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1/100,000 dilution was used for 1 hour at room temperature.

image 2 :

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue with Mouse anti-DAP Kinase 1 antibody (HA601162) at 1/2,000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA601162) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

product information

SKU :

HA601162

Target name :

DAP Kinase 1

Species reactivity :

Human

Applications :

WB,IHC-P

Conjugate :

Non-conjugated

Immunogen :

Recombinant protein within human DAP Kinase 1 aa 451-700 / 1,430.

Uniprot id :

P53355>SwissProt: P53355 Human

Host :

Mouse

Clone number :

12B2-R

Isotype :

IgG1

Size :

100μl

List Price :

360.00 USD

Storage Buffer :

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Form :

Liquid

Storage Instruction :

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.

Purity :

Protein A affinity purified.

Product type :

Recombinant Mouse monoclonal Antibody

Positive control :

A549 cell lysate, TF-1 cell lysate, HepG2 cell lysate, human brain tissue lysate, human lung carcinoma tissue.

Molecular wt :

Predicted band size: 160 kDa

Subcellular location :

Cytoplasm, cytoskeleton.

Concentration :

1 mg/mL.

Recommended dilutions :

WB: 1:2,000 ;IHC-P: 1:2,000

more info or order :

HUABIO product webpage